Microbiological Analysis and Metagenomic Profiling of the Bacterial Community of an Anthropogenic Soil Modified from Typic Haploxererts

This work aimed to characterize the microbial communities of an anthropogenic soil originating from application pedotechniques Vertisols in a Mediterranean environment. Bare profiles were sampled at three depths (0–10 cm, 10–30 and 30–50 cm) compared with original not transformed same depths. The soils characterized by higher CaCO3 concentration (360–640 g/kg) than control (190–200 g/kg), while opposite trend was registered for clay, where showed (465 g/kg on average) (355 average). Organic carbon content much untransformed soil. All samples microbiologically investigated using combined culture-dependent -independent approach. Each pedon displayed generally decreasing level depth several groups investigated; particular, filamentous fungi below detection limit cm. To isolate bacteria actively involved particle aggregation, colonies mucoid appearance differentiated strain genetically identified: major represented Bacillus Pseudomonas. MiSeq Illumina analysis identified Actinobacteria Firmicutes as main groups. A high variability found all pedons microorganisms constitute mature community.